We constructed terpene high yield chassis (E. coli, Saccharomyces cerevisiae, Aspergillus oryzae), provided sufficient substrate for terpene synthase, achieved efficient synthesis of terpene products, significantly reduced the time and cost of functional identification of terpene synthase, and efficiently excavated and elucidated the cyclization mechanism and enzyme catalytic mechanism of a series of new skeletal terpene products.

In this study, we found for the first time that filamentous fungi derived type I chimeric terpenoid synthases TvTS and MpMS can directly synthesize triterpenoid core skeleton using IPP, DMAPP or HexPP as substrates. Subsequently, the synthesis of triterpenoid skeleton was proved to be the natural property of this enzyme by in vitro reaction and homologous activation experiments. Then, the absolute configuration and cyclization mechanism of the products were analyzed by in vitro isotope feeding e...

We have developed an innovative mining strategy of terpenoid products "based on a microbial high-yield chassis and biofoundry platform", to achieve batch mining of terpene synthases and gene clusters, effectively solving the bottleneck of low throughput. It provides a complete and efficient solution for this field, including "gene cluster mining, activity screening, biosynthesis mechanism analysis, and efficient product synthesis"and other processes