We constructed terpene high yield chassis (E. coli, Saccharomyces cerevisiae, Aspergillus oryzae), provided sufficient substrate for terpene synthase, achieved efficient synthesis of terpene products, significantly reduced the time and cost of functional identification of terpene synthase, and efficiently excavated and elucidated the cyclization mechanism and enzyme catalytic mechanism of a series of new skeletal terpene products.
