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系统管理员 2025-04-28 19:07:06

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系统管理员 2025-04-28 19:05:28

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系统管理员 2025-04-28 19:04:07

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中国科学院深圳先进技术研究院甘海云研究员课题组招聘单位介绍：中国科学院深圳先进技术研究院合成生物学研究所 (http://isynbiosiataccn/) 成立于2017年12月。合成所采用合成生物学的工程化设计理念，专注于人造生命元件、基因线路、生物器件、多细胞体系等的合成再造研究，旨在揭示生命本质和探索生命活动基本规律研究所积极开展面向市场的、以产业化为导向的技术转化，力求发展成为国际上具影响力的合成生物学研发基地与产业创新中心。研究所通过打造开放交叉合作的平台，汇聚国内外合成生物学领域青年骨干及海内外领军科学家，打造一支年轻有活力、多学科融合的前沿创新团队。 PI介绍及研究方向：（课题组网站:http://isynbiosiataccn/ganlab/）甘海云，现任中国科学院深圳先进技术研究院研究员。2011年在中国科学院动物研究所获得博士学位，之后先后在美国 mayo clinic 以及哥伦比亚大学进行博士后研究, 主要研究领域是表观基因组信息的遗传机制以及肿瘤发生和耐药性产生的表观遗传调控机制。回国前是美国ST JUDE儿童研究医院细胞分子生物学部门生物信息负责人。已在表观基因组领域取得了一系列重要的成果。共发表 SCI 论文 36 篇，累计影响因子>440, 累计他引>3000次，其成果发表在包括Cell, Science (2篇)、Nature Genetics,Molecular Cell (4篇)、PNAS、NAR、Nature Communications、eLife、Mol Cell Proteomics等国际专业期刊上。在这个坚实的基础上，申请人将和PI一起结合合成生物学在这个领域进行更深入的机制和功能研究。主要研究方向：课题组主要利用系统生物学、合成生物学研究表观基因组信息的传递机制、肿瘤发生和耐药性产生的表观遗传调控机制、衰老的表观遗传调控机制和表观抗衰老药物的筛选、构建基因线路延缓衰老。一、招聘岗位：研究助理（2-3名）岗位亮点： l一流科研平台 l充足经费保障 l学科交叉引领 l开放产业环境 l多维发展路径 l3H全方位保障职位月薪：8K-12K元/月工作性质：全职工作经验：不限最低学历：硕士招聘人数：2-3人（一）岗位描述在PI指导下，独立开展研究工作或者加入研究小组，和其他成员合作完成课题。表现突出的员工，2个课题组支持读博深造。（二）应聘要求 1硕士及以上学历；具有合成生物学、系统生物学、生物信息学、肿瘤生物学、生物化学与分子生物学、细胞生物学、表观基因组学等背景，或具有组学实验技术(如ChIP-Seq、cut&tag、单细胞测序、临近标记蛋白组学)、定量质谱、细胞培养操作、小鼠实验、合成基因组技术、分子载体构建等相关研究经验者优先考虑。 2团队合作意识强，具有认真负责的工作态度，积极创新的科研热情。（三）薪资福利 1．竞争力薪酬+绩效奖励+年终奖金； 2．稳定聘期+晋升空间； 3．人才安居房+高端体检+定点医院VIP服务+子女入学； 4．全额缴纳五险一金+工会福利+工作餐补+带薪年假； 5．国家级科研前辈一对一指导，专业团队协助申请各级补贴及各类项目； 6．提供国际领先的科研条件，包括良好的工作环境、充足的研究经费、前沿的实验仪器和愉悦工作氛围。（四）工作地点深圳市光明区永创路108号。（五）应聘方式有意申请者请将个人简历（要求为PDF）发送至hygan@siataccn，简历及邮件标题注明“应聘岗位-学校名称-专业-姓名”。

研究合成 生物学 工作技术

系统管理员 2025-04-28 19:00:57

Patents

甘海云，文青，李欣然，周嘉琦，中国发明专利，“临近标记复合物、临近标记方法、分子间互作分析方法"(专利申请号:CN2021109952378；PCT/CN2021/114989)

专利方法 CN 标记临近

系统管理员 2025-04-28 18:59:29

Papers

Xing Kang#,Xinran Li#,Jiaqi Zhou#,Yang Zhang#,Lingyu Qiu#,Congcong Tian#,Zhiwen Deng#, Xiaoyan Liang, Ziwei Zhang, Songlin Du, Suili Hu, Nan Wang, Zhen Yue, Yajing Xu, Yuan Gao, Junbiao Dai, Zhiquan Wang, Chuanhe Yu, Jinyi Chen, Yuchun Wu, Liangming Chen, Yuan Yao, Sitong Yao, Xinran Yang, Lixia Yan, Qing Wen, Olivia M Depies, Kuiming Chan， Xiaohuan Liang, Gang Li, Zhike Zi, Xiangyu Liu,Haiyun Gan*,Extrachromosomal DNA Replication and Maintenance Couple with DNA Damage Pathway in Tumors,Cell(2025) Sitong Yao, Zhen Yue, Shaotang Ye , Xiaohuan Liang, Yugu Li*,Haiyun GanandJiaqi Zhou*Identification of MCM2-Interacting Proteins Associated with Replication Initiation Using APEX2-Based Proximity Labeling TechnologyInt J Mol Sci（2025） Sr iniv asu Kar r i , Yi Yang,Jiaqi Zhou, Quinn Dickinson, Jing Jia, Yuxin Huang, Zhiquan Wang,Haiyun Gan, * and Chuanhe Yu* “Defective transfer of parental histone decreases frequency of homologous recombination by increasing free histone pools in budding yeast”Nucleic Acids Research, （2024） CongcongTian#,QinZhang#,JingJia#,JiaqiZhou#,ZiweiZhang#,SrinivasuKarri,JiuhangJiang,QuinnDickinson,YuanYao,XiaorongTang,YuxinHuang,TingGuo,ZiweiHe,ZhengLiu,YuanGao,XinranYang,YuchunWu,KuimingChan,DaoqinZhang,JunhongHan*,ChuanheYu*,andHaiyunGan* "DNA polymerase delta governs parental histone transfer to DNA replication lagging strand"PNAS(2024) Congcong Tian#,Jiaqi Zhou#,Xinran Li#, Yuan Gao, Qing Wen, Xing Kang, Nan Wang, Yuan Yao, Jiuhang Jiang, Guibing Song, Tianjun Zhang, Suili Hu, JingYi Liao, Chuanhe Yu, Zhiquan Wang, Xiangyu Liu, Xin-hai Pei, Kuiming Chan, Zichuan Liu &Haiyun Gan* "Impaired histone inheritance promotes tumor progression"Nature Communications , (2023) Qing Wen#,Jiaqi Zhou#,Congcong Tian#,Xinran Li#,Guibing Song#,Yuan Gao, Yaping Sun, Chiyuan Ma, Sitong Yao, Xiaoyan Liang, Xing Kang, Nan Wang, Yuan Yao, Hongbao Wang, Xiaohuan Liang, Jialin Tang, Steven M Offer, Xiaohua Lei, Chuanhe Yu, Xiangyu Liu, Zichuan Liu, Zhiquan Wang, andHaiyun Gan*,‘‘Symmetric inheritance of parental histonescontributes to safeguardingthe fate of mouse embryonic stem cells during differentiation’Nature Genetics ,（2023） Yao Y#,Wen Q#,Zhang T, Yu C, Chan KM,Gan H* "Advances in Approaches to Study Chromatin-Mediated Epigenetic Memory"ACS Synth Biol doi: 101021/acssynbio1c00394(2021) Albert Serra-Cardona, Chuanhe Yue , Xinmin Zhang , Xu Hua,Yuan Yao,Jiaqi Zhou,Haiyun Gan*, and Zhiguo Zhang*, "A mechanism for Rad53 to couple leading- and lagging-strand DNA synthesis under replication stress in budding yeast"PNAS,118: e2109334118(2021) Xinran Li#,Jiaqi Zhou#,Wenjuan Zhao#,Qing Wen#,Weijie Wang, Huipai Peng, Yuan Gao, Kelly J Bouchonville, Steven M Offer, Kuiming Chan, Zhiquan Wang*,Nan Li*,Haiyun Gan*"Defining proximity proteomics of histone modifications by antibody-mediated protein A-APEX2 labeling"Genomics, Proteomics & Bioinformatics, (2021) QWen#,Y Yao#,XLi, ZHu, H Mei,HGan*"Chromatin replication andparental histone allocation"Genome Instability & Disease2:51–58(2021)

Gan Yuan Yao Wang histone

系统管理员 2025-04-28 18:59:04

Papers

Yu, C#,H Gan#, A Serra-Cardona, L Zhang, S Gan, S Sharma, E Johansson, A Chabes, R M Xu and Z Zhang "A mechanism for preventing asymmetric histone segregation onto replicating DNA strands"Science 361, 1386-1389(2018) (Highlighted in Science and F1000) Li, S, Z Xu, J Xu, L Zuo, C Yu, P Zheng,H Gan, X Wang, L Li and S Sharma "Rtt105 functions as a chaperone for replication protein A to preserve genome stability"The EMBO journal: e99154 (2018) H Gan#, A Serra-Cardona#, X Hua, H Zhou, K Labib, C Yu and Z ZhangThe Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging StrandsMol Cell72(1): 140-151 e143 (2018) Fang, D#,H Gan#, L Cheng, J H Lee, H Zhou, J N Sarkaria, D J Daniels and Z ZhangH33K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancersELife,7 (2018) H Gan#, C Yu#, S Devbhandari, S Sharma, J Han, A Chabes, D Remus and Z Zhang*,Checkpoint kinase Rad53 couples leading and lagging strand DNA synthesis under replication stressMol Cell68, 446-455 e443 (2017) (Highlighted in CellCycle) Zhang, H#,H Gan#, Z Wang#, J H Lee, H Zhou, T Ordog, M S Wold, M Ljungman and Z Zhang*RPA Interacts with HIRA and Regulates H33 Deposition at Gene Regulatory Elements in Mammalian CellsMol Cell,65(2):272-284 (2017) (Highlighted in F1000) Fang, D#,H Gan#, J H Lee#, J Han#, Z Wang#, S M Riester, L Jin, J Chen, HZhou, J Wang, H Zhang, N Yang, E W Bradley, T H Ho, B P Rubin, J A Bridge, S N Thibodeau, T Ordog, Y Chen, A J van Wijnen, A M Oliveira, R M Xu, J JWestendorf and Z Zhang*,The histone H33K36M mutation reprograms the epigenome of chondroblastomasScience352, 1344-1348 (2016) (Highlighted inF1000) C Yu#,H Gan#and Z Zhang*Both DNA Polymerases delta and Contact Active and Stalled Replication Forks differentlyMol Cell Biol,37(21) (2017) Feng, J#,H Gan#, M L Eaton, H Zhou, S Li, J A Belsky, D M MacAlpine, Z Zhang* and Q Li*,Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant CellsMol Cell Biol36, 1856-1867 (2016) C Yu#,H Gan#, J Han, Z X Zhou, S Jia, A Chabes, G Farrugia, T Ordog, Z Zhang*Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks StallMol Cell, 56, 551-563 (2014) (Highlighted in Molecular Cell and F1000) H Gan#, L Wen#, S Liao#, X Lin, T Ma, J Liu, C X Song, M Wang, C He*, C Han*, F Tang*Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesisNat Commun4, 1995 (2013) H Gan#, T Cai#, X Lin, Y Wu, X Wang, F Yang*, C Han*Integrative Proteomic and Transcriptomic Analyses Reveal Multiple Post-transcriptional Regulatory Mechanisms of Mouse SpermatogenesisMol Cell Proteomics, 12, 1144-1157 (2013) H Gan, X Lin, Z Zhang, W Zhang, S Liao, L Wang, C Han*piRNA profiling during specific stages of mouse spermatogenesisRNA, 17, 1191-1203 (2011) Co-author Hashizume, R, N Andor, Y Ihara, R Lerner,H Gan, X Chen, D Fang, X Huang, M W Tom and V Ngo "Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma"Nature medicine20(12): 1394 (2014) Chen, X, M Zhang,H Gan, H Wang, J-H Lee, D Fang, G J Kitange, L He, Z Hu and I F Parney "A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma"Nature communications9(1): 2949 (2018) Gao, Y,H Gan, Z Lou and Z Zhang "Asf1a resolves bivalent chromatin domains for the induction of lineage-specific genes during mouse embryonic stem cell differentiation"PNAS115(27): E6162-E6171 (2018) Li, S, Z Xu, J Xu, L Zuo, C Yu, P Zheng,H Gan, X Wang, L Li and S Sharma "Rtt105 functions as a chaperone for replication protein A to preserve genome stability"The EMBO journal: e99154 (2018) Wu, R, Z Wang, H Zhang,H Ganand Z Zhang "H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication"Nucleic acids research45(1): 169-180 (2016) Chan, K M, J Han, D Fang,H Ganand Z Zhang "A lesson learned from the H3 3K27M mutation found in pediatric glioma: a new approach to the study of the function of histone modifications in vivo?"Cell cycle12(16): 2546-2552 (2013) Fang, D, HGan, H Wang, H Zhou and Z Zhang"Probe the function of histone lysine 36 methylation using histone H3 lysine 36 to methionine mutant transgene in mammalian cells"Cell Cycle16(19): 1781-1789 (2017) Hou, X, W Zhang, Z Xiao,H Gan, X Lin, S Liao and C Han "Mining and characterization of ubiquitin E3 ligases expressed in the mouse testis"BMC genomics13(1): 495 (2012) Yang, Y, Y Feng, X Feng, S Liao, X Wang,H Gan, L Wang, X Lin and C Han "BMP4 cooperates with retinoic acid to induce the expression of differentiation markers in cultured mouse spermatogonia"Stem cells international(2016) Yang, Y, M Leonand andH Gan "Viral and Nonviral Cancer Gene Therapy THE WORLD SCIENTIFIC ENCYCLOPEDIA OF NANOMEDICINE AND BIOENGINEERING I: Volume 3: Nanotechnology in Gene Delivery",World Scientific:1-52 (2017) Yu, C,H Ganand Z Zhang "Strand-Specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding YeastGenome Instability, Springer: 227-238 (2018) L Cheng, X Zhang, Y Wang,H Gan, X Xu, X Lv, X Hua, J Que, T Ordog "Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit"Nucleic acids research47 (21), 11114-11131 (2019) JS Lee, Y Mo,H Gan, RJ Burgess, DJ Baker, JM van Deursen, Z Zhang"Pak2 kinase promotes cellular senescence and organismal aging"Proceedings of the National Academy of Sciences116 (27), 13311-13319 (2019) SW Yang, L Li, JP Connelly, SN Porter, K Kodali,H Gan, JM Park "A cancer-specific ubiquitin ligase drives mRNA alternative polyadenylation by ubiquitinating the mRNA 3′ end processing complex"Molecular Cell77 (6), 1206-1221 e7 （2020） Chan, K M, J Han, D Fang,H Ganand Z Zhang "A lesson learned from the H3 3K27M mutation found in pediatric glioma: a new approach to the study of the function of histone modifications in vivo?"Cell cycle12(16): 2546-2552(2013)

Zhang Cell Gan

系统管理员 2025-04-28 18:53:36

Research

Unlocking the Epigenetic Code: Synthetic Biology Meets Chromatin Engineering Emerging insights into epigenetic regulation have revealed chromatin-associated pathways as master conductors of cellular identity, aging, and specialization Our laboratory sits at the cutting edge of this revolution, uniquely bridging synthetic biology with epigenetic engineering to reimagine how we understand and manipulate life's molecular control systems We pursue three transformative research frontiers: 1 Designing Epigenetic Control Modules We hunt for nature's hidden toolbox – discovering novel chromatin regulators (including DNA/histone modifiers and epigenetic "switches") – then rewire them into synthetic circuits Like building molecular dimmer switches for genes, we engineer these components to create programmable systems that precisely tune chromatin states, opening new possibilities for controlling cellular behavior 2 Building Smart Cell Factories Imagine microbes or mammalian cells with upgradable "epigenetic software"! We redesign chromatin architecture in industrial cells using synthetic pathways, achieving two breakthroughs: Supercharged Bioproduction: Lock cells into high-performance metabolic states for sustainable chemical synthesis Living Epigenetic Labs: Create cellular models to crack unanswered questions in development and disease 3 Precision Editing of Cellular Memory We pioneer next-generation tools that edit epigenetic marks without altering DNA Our dCas9-based editors act like molecular erasers and pens for chromatin, currently being optimized to: Reset cancer cells by wiping out harmful epigenetic "memories" driving tumor growth Develop safer epigenetic therapies that preserve genome integrity

epigenetic We chromatin cells cellular

系统管理员 2025-04-28 18:44:01

Research

Extrachromosomal DNA (ecDNA) drives the evolution of cancer cells However, the functional significance of ecDNA and the molecular components involved in its replication and maintenance remain largely unknown Here, using CRISPR-C technology, we generated ecDNA-carrying (ecDNA+) cell models By leveraging these models alongside other well-established systems, we demonstrated that ecDNA can replicate and be maintained in ecDNA+ cells The replication of ecDNA activates the ATM-mediated DNA damage response (DDR) pathway Topoisomerases, such as TOP1 and TOP2B, play a role in ecDNA replication-induced DNA double strand breaks (DSBs) A subset of these elevated DSBs persists into the mitotic phase and is primarily repaired by the alternative non-homologous end joining pathway (alt-NHEJ), which involves POLθ and LIG3 Correspondingly, ecDNA maintenance requires DDR, and inhibiting DDR impairs the circularization of ecDNA

ecDNA DNA pathway maintenance DDR

系统管理员 2025-04-28 18:43:07

Administrative Assistants

Wang 王茜 Qian

系统管理员 2025-04-25 15:59:52