Cells are evolved systems optimized for survival and growth, which often conflicts with their engineered function as “cell factories” for target chemical production. Product synthesis typically imposes metabolic burden and stress, constraining performance. We integrate classical metabolic engineering with laboratory evolution, employing strategies such as growth‑production coupling and orthogonal pathway design to systematically reshape metabolic networks. Our goal is to construct robust cell factories that maintain metabolic homeostasis while achieving high‑level production.
 

细胞代谢的重塑与维持
细胞是自然进化形成的、以生存与生长为优化目标的复杂系统。当将其改造为用于合成的“细胞工厂”时，目标产物的积累往往带来代谢压力与负荷，限制其性能。我们通过整合传统代谢工程策略与实验室进化手段，基于“生长‑生产偶联”或“正交化代谢”等逻辑，对细胞代谢网络进行系统性重塑，旨在构建以高效生产为最优目标、且代谢稳态得以维持的细胞工厂。