Representative Publications

# Equal contribution, * Corresponding author

2024

                                                                                                                                                                                                                                                                                                

62.Yanyi Wang#, Qian Zhang#, Changhao Ge, Bolin An, Chao Zhong*. Programmable Bacterial Biofilms as Engineered Living Materials. Accounts of materials research, 2024,https://pubs.acs.org/doi/10.1021/accountsmr.3c00271

61.Xinyu Wang#, Boyang Zhang, Jicong Zhang, Xiaoyu Jiang, Kaiwei Liu, Haifeng Wang, Xinyi Yuan, Haiyi Xu, Yijun Zheng, Guijun Ma*, and Chao Zhong*. Conformal and conductive biofilm-bridged artificial Z-scheme system for visible light-driven overall water splitting. Science advances,2024,https://doi.org/10.1126/sciadv.adn6211

60.Jun Liang#, Kemeng Xiao, Xinyu Wang, Tianfeng Hou, Cuiping Zeng*, Xiang Gao*, Bo Wang*, and Chao Zhong*. Revisiting Solar Energy Flow in Nanomaterial-Microorganism Hybrid Systems.Chemical reviews,2024,https://doi.org/10.1021/acs.chemrev.3c00831

2023

                                                                                                                                                                                                                                                                                           

59.Yi Liu#, Mengyao Wu, Anqi Kang, Xiehaoran Zhang, Ronghui Xie, Yuanpei Huang,Jiaofang Huang, Lien-Yang Chou,* and Chao Zhong*,Living Seed Materials Made by Metal–Organic Framework-Encapsulated Bacillus Subtilis Spore,Advanced Functional Materials,2023,DOI:10.1002/adfm.202309288

58.Yuanyuan Huang^#, Yanfei Wu , Han Hu , Bangzhuo Tong , Jie Wang, Siyu Zhang , Yanyi Wang, Jicong Zhang, Yue Yin  , Shengkun Dai, Wenjuan Zhao , Bolin An1,2, Jiahua Pu, Yaomin Wang, Chao Peng , Nan Li , Jiahai Zhou* ,Yan Tan *& Chao Zhong*.Accelerating the design of pili-enabled living materials using an integrative technological workflow. Nature Chemical Biology,2023,https://doi.org/10.1038/s41589-023-01489-x

57.Rational design of functional amyloid fibrillar assemblies, Xinyu Wang#, Shengnan Zhang#, Jicong Zhang,Yaomin Wang, Xiaoyu Jiang, Youqi Tao, Dan Li, Chao Zhong* and Cong Liu*,Chemical Society Reviews, 2023.https://pubs.rsc.org/en/content/articlelanding/2023/cs/d2cs00756h/unauth

56.Effective disposal and remediation of chemical agents with designer living biofifilm materials in soil and water.Xuan Guo, Suying Liu, Lei Zhou, Bin Xue, Yongpeng Huang,Junwen Li, Jinyi Zhong, Chao Zhong.Fundamental Research,2023.

 

55.Surface Wrinkling with Memory for Programming Adhesion and Wettability.Feng Wang, Senbo Xiao, Sihai Luo, Yuequn Fu, Bjo Helge Skallerud, Helge Kristiansen, Mengkui Cui,Chao Zhong, Siqi Liu, Yizhi Zhuo, Jianying He,* and Zhiliang Zhang*.ACS Applied Nano Materials.2023,6(6)4097-4104.https://doi.org/10.1021/acsanm.2c05410

54.Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration.Hongping Yu, Yanyi Wang, Junjie Gao , Youshui Gao *, Chao Zhong*, & Yixuan Chen*.Communications Biology 6 (1),197.https://doi.org/10.1038/s42003-023-04567-x

53.Engineered living materials grown from programmable Aspergillus niger mycelial pellets.Ke Li, Zhen Wei, Jianyao Jia, Qing X , Hao Liu, Chao Zhong, He Huang.Materials Today Bio.19 (2023) 100545

52.Full-colour Jabuticaba-like nanostructures via the multiplex and orthogonal self-assemblyof protein-coniugated quantum dots with engineered biofilms.Materials Horizons 2023, 10(4), 1440-1445. DOI: 10.1039/D2MH01231F

51.Engineered Living Materials For Sustainability.Bolin An, Yanyi Wang, Yuanyuan Huang, Xinyu Wang, Yuzhu Liu, Dongmin Xun,George M. Church, Zhuojun Dai,* Xiao Yi,* Tzu-Chieh Tang,* and Chao Zhong*.Chemical Reviews.2023, 123, 2349−2419.

50..Suying Liu#, Jiaofang Huang#* , Chen Zhang, Lihua Wang, Chunhai Fan, and Chao Zhong*. Probing the Growth and Mechanical Properties of Bacillus subtilis Biofilms through Genetic Mutation Strategies. Synthetic and Systems Biotechnology.2023,https://doi.org/10.1016/j.synbio.2022.05.005. (Q1).

49.Establishment of a salt-induced bioremediation platform from marine Vibrio natriegens.Ling Huang , Jun Ni*, Chao Zhong , Ping Xu  , Junbiao Dai & Hongzhi Tang*.CommunicationsBiology.2022,5(1).1352.https://doi.org/10.1038/s42003-022-04319-3

48.Rational Design of a Zr-MOF@ Curli-Polyelectrolyte Hybrid Membrane toward EfficientChemical Protection, Moisture Permeation, and Catalytic Detoxification. Jing Liu, Heguo Li, Bin Yan, Chao Zhong, Yue Zhao,* Xuan Guo,* and Jinyi Zhong*. ACS Applied Materials & Interfaces.2022,14 (47)53421-53432

47.Yanyi Wang^#;Yi Liu^#;Jing Li; Yue Chen; Sizhe Liu ;Chao Zhong*.Engineered Living Materials (ELMs) Design: From Function Allocation to Dynamic Behavior Modulation.Current Opinion in Chemical Biology,2022,https://doi.org/10.1016/j.cbpa.2022.102188.

46.Xiaokang Zhang#, Mengkui Cui#, Shuoshuo Wang#, Fei Han#, Pingping Xu , Luyao Teng,Hang Zhao, Ping Wang, Guichu Yue, Yong Zhao , Guangfeng Liu, Ke Li, Jicong Zhang,Xiaoping Liang , Yingying Zhang , Zhiyuan Liu*, Chao Zhong*,& Weizhi Liu*,Extensible and self-recoverable proteinaceous materials derived from Scallop Byssal Thread, Nature Communications,2022,https://www.nature.com/articles/s41467-022-30415-3

45.Yuanyuan Huang, Mingyi Zhang, Jie Wang, Dake Xu,and Chao Zhong*, Engineering microbial systems for the production and functionalization of biomaterials,Current Opinion in Microbiology,2022,https://doi.org/10.1016/j.mib.2022.102154

43.X. Y. Wang#, J. C. Zhang#, et al., C. Zhong*, Photocatalyst-mineralized biofilms as living bio-abiotic interfaces for single enzyme to whole-cell photocatalytic applications. Science Advances 2022. 7665 (2022)

42.Xiaoyu Jiang, Teng Feng, Bolin An,Susu Ren, Jufeng Meng, Ke Li, Suying Liu, Haiying Wu, Hui Zhang,* and Chao Zhong* A Bi-Layer Hydrogel Cardiac Patch Made of Recombinant Functional Proteins. Advanced Materials, 2022, https://doi.org/10.1002/adma.202201411

41.Kemeng Xiao,^# Jun Liang,^# Xinyu Wang,^# Tianfeng Hou, Xiaoning Ren, Panqing Yin, Zhiping Ma, Cuiping Zeng, Xiang Gao, Tao Yu, Tong Si, Bo Wang,* Chao Zhong,* Zhifeng Jiang,* Chun-Sing Lee, Jimmy Chai-mei Yu  and Po Keung Wong, Panoramic insights into semi-artificial photosynthesis: origin, development, and future perspective. Energy & Environmental Science , 2022, 15 (2), 529-549.

40.Kai Zhou,^# Yang Liu,^# Ke Li,^# Yingfeng Li, Shicao Wei, Yule Zhou, Hong Yu, Yanru Zhang, Jingqi Lv, Botao Xiao,* and Chao Zhong*, Force Spectra of a Single CsgA Molecule and Amyloid Nanofibers Assembled from Chimeric Mfp5 and CBD Proteins: Implications for a Nanomaterial Testing Machine. ACS Applied Nano Materials，2022, 5 (2), 1758-1766.

39.Li, Zhong^#; Wang, Xinyu^#; Wang, Jie; Yuan, Xinyi; Jiang, Xiaoyu; Wang, Yanyi; Zhong, Chao*; Xu, Dake*; Gu, Tingyue; Wang, Fuhui, Bacterial biofilms as platforms engineered for diverse applications. Biotechnology Advances, 2022, 57, 107932.

38.Shicao Wei; Yingfeng Li; Ke Li; Anqi Kang; Siyu Zhang; Teng Feng; Hui Zhang; Chao Zhong*. Functional amyloid-chitin hybrid ink coupled with flexible fabrication approaches for diverse macro and micro-structures.Materials Today Bio,2022, https://doi.org/10.1016/j.mtbio.2021.100179

2021

                                                                                                                                                                                                                                                                                           

37.Meixia Liu, Pingping Han, Lingling Zhang, Chao Zhong, and Chun You*, Biofilm-Mediated Immobilization of a Multienzyme Complex for Accelerating Inositol Production from Starch. Bioconjugate Chemistry ，2021, 32 (9), 2032-2042.

36.Tzu-Chieh Tang^#; Bolin An^#; Yuanyuan Huang; Sangita Vasikaran; Yanyi Wang; Xiaoyu Jiang; Timothy K. Lu* & Chao Zhong*. Materials design by synthetic biology. Nature Reviews Materials, 2021, https://doi.org/10.1038/s41578-020-00265-w.

35.Lianjie Xu; Xinyu Wang; Fei Sun; Yi Cao; Chao Zhong*; Wen-Bin Zhang*. Harnessing proteins for engineered living materials. Current Opinion in Solid State and Materials Science, 2021, 25 (1), 100896.

2020

                                                                                                                                                                                                                                                                                            

34.Yanyi Wang^#; Bolin An^#; Bin Xue; Jiahua Pu; Xiuli Zhang; Yuanyuan Huang; Yi Yu; Yi Cao; Chao Zhong*. Living materials fabricated via gradient mineralization of light-inducible biofilms. Nature Chemical Biology, 2020, https://doi.org/10.1038/s41589-020-00697-z

33.Bolin An^#; Yanyi Wang^#; Xiaoyu Jiang^#; Conghui Ma; Mark Mimee; Felix Moser; Ke Li; Xinyu Wang; Tzu-Chieh Tang; Yuanyuan Huang; Yifan Liu; Timothy K.Lu; Chao Zhong*. Programming Living Glue Systems to Perform Autonomous Mechanical Repairs. Matter, 2020, 3 (6), 2080-2092.

32.Ke Li^#; Yingfeng Li^#; Xinyu Wang; Mengkui Cui; Bolin An; Jiahua Pu; Jintao, Liu; Boyang Zhang; Guijun Ma; Chao Zhong*. Diatom-inspired multiscale mineralization of patterned protein-polysaccharide complex structures. National Science Review, 2020, nwaa191.

31.J. Pu^#, Y. Liu^#, J. Zhang, B. An, Y. Li, X.Wang, K. Din, C. Qin, K. Li, M. Cui, S. Liu, Y. Huang, Y. Wang, Y.Lv, J. Huang, Z. Cui, S. Zhao, C. Zhong^＊,“Virus disinfection from environmental water sources using living engineered biofilm materials” Advanced Science 2020，(Accepted) DOI:10.1002/advs.201903558

30.Y. Li^#, K. Li^#, X. Wang, M. Cui, P. Ge, J. Zhang, F. Qiu, C. Zhong*, Conformable Self-assembling Amyloid Protein Coatings with Genetically Programmable Functionality,Science Advances 2020  (In press) DOI:10.1126/sciadv.aba1425

29.Q. Wang, C. Zhong* and H. Xiao*, Genetic engineering of filamentous fungi for efficient protein expression and secretion, Frontiers in Bioengineering and Biotechnology 2020,8,293. DOI: 10.3389/fbioe.2020.00293

28.C. Zhong*，Spores hit the spot, Nature Chemical Biology, 2020, 16, 108-109. DOI: 10.1038/s41589-019-0451-y. (News and views)

2019

                                                                                                                                                                                                                                                                                       

27.L. Nie^#, Y. Li^#, S. Chen, K. Li, Y. Huang, Y. Zhu, Z. Sun, J. Zhang, Y. He, M. Cui, S. Wei, F. Qiu, C. Zhong*, and W. Liu*, Biofilm Nanofiber-Coated Separators for Dendrite-Free Lithium Metal Anode and Ultrahigh-Rate Lithium Batteries, ACS Appl. Mater. Interfaces, 2019, 11, 35, 32373-32380. DOI: 10.1021/acsami.9b08656

26.Y. Li, C. Zhong* et al, Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality, Nano Letters, 2019. DOI: 10.1021/acs.nanolett.9b02324

25.F. Tan^#*, C. Chu^#, J. Qi^#, W. Li^#, D. You, K. Li, X. Chen, W. Zhao, C. Cheng, X. Liu, Y. Qiao, B. Su, S. He, C. Zhong, H. Li*, R. Chai* & G. Zhong*, AAV-ie enables safe and efficient gene transfer to inner ear cells, Nature Communications, 2019, 10, 3733. DOI: 10.1038/s41467-019-11687-8

24.X. Wang, C. Zhong* et al, Immobilization of Functional Nano-objects in Living Engineered Bacterial Biofilms for Catalytic Applications, National Science Review, nwz104, DOI: org/10.1093/nsr/nwz104.

23.M. Cui, C. Zhong* et al, Exploiting Mammalian Low Complexity Domains for Liquid-liquid Phase Separation Driven Underwater Adhesive Coatings, Science Advances, 2019, 5(8), eaax3155.

22.Y. Liu^#, J. Qi^#, X. Chen^#, M. Tang^#, C. Chu, W. Zhu, H. Li, C. Tian, G. Yang, C. Zhong, Y. Zhang, G. Ni, S. He*, R. Chai* and G. Zhong*, Critical role of spectrin in hearing development and deafness, Science Advances, 2019, 5(4), eaav7803. DOI: 10.1126/sciadv.aav7803.

21.X. Mao, K. Li, M. Liu, X. Wang, T. Zhao, B. An, M. Cui, Y. Li, J. Pu, J. Li, L. Wang, T. K. Lu, C. Fan*, and C. Zhong*. Directing Curli Polymerization with DNA Origami Nucleators. Nature communications 2019, 10, 1395. DOI: 10.1038/s41467-019-09369-6.

20.M. Cui^#, Q. Qi^#, T. Gurry^#, T. Zhao,B. An , J. Pu, X. Gui, A. Cheng, S. Zhang, D. Xun, M. Becce, F. Vangosa, C. Liu, T. K. Lu, C. Zhong* Genetic Design of Multi-domain Functional Amyloids: Insights into Self-assembly and Functional Properties, Chemical Science 2019, 10, 4004-4014. DOI: 10.1039/C9SC00208A.

19.J. Huang^#, S. Liu^#, C. Zhang^#, X. Wang, J. Pu, F. Ba, S. Xue, H. Ye, T. Zhao, K. Li, Y. Wang, J. Zhang, L. Wang, C. Fan, T. K. Lu, C. Zhong*, Programmable and Printable Bacillus subtilis Biofilms as Engineered Living Materials. Nature Chemical Biology 2019, 15, 34–41.

18.C. Zhang^#, J. Huang^#, J. Zhang^#, S. Liu, M. Cui, B. An, X. Wang, J. Pu, T. Zhao, C. Fan, T. K. Lu, C. Zhong*, Engineered Bacillus subtilis biofilms as living glues, Materials Today 2019, 28, 40-48. DOI: 10.1016/j.mattod.2018.12.039.

Before 2019

                                                                                                                                                                                                                                                                                                 

17.X. Wang^#, J. Pu^#, B. An^#, Y. Li,Y. Shang, Z. Ning, Y. Liu, F. Ba,J. Zhang, C. Zhong*, Programming cells for dynamic assembly of inorganic nano-objects with spatiotemporal control. Advanced Materials 2018, 30, 1705968. (Inside back cover & video abstract highlight)

16.C. Zhang^#, Y. Li^#, H. Wang, S. He, Y. Xu, C. Zhong* and T. Li*, Adhesive bacterial amyloid nanofiber-mediated growth of metal–organic frameworks on diverse polymeric substrates. Chemical Science 2018, 9, 5672–5678.

15.Y. Wang^#, J. Hua^#, B. An^#, T. K. Lu*, C. Zhong*, Emerging Paradigms for Synthetic Design of Functional Amyloids, Journal of Molecular Biology 2018 in press, (Invited review in theme issue “functional amyloids”).

14.L. Jiang, X. Song, Y. Li, Q. Xu, J. Pu, H. Huang*, and C. Zhong*, Programming integrative extracellular and intracellular biocatalysis for rapid, robust, and recyclable synthesis of trehalose. ACS Catalysis 2018, 8, 1837−1842.

13.Q. Qi, T. Zhao, B. An, X. Liu, C. Zhong*, Self-assembly and morphological characterization of two-component functional amyloid proteins, Chinese Chemical Letters, 2017, 28(5), 1062-1068. DOI: 10.1016/j.cclet.2016.12.008

12.B. An^#, X. Wang^#, M. Cui^#, X. Gui, X. Mao, Y. Liu, K. Li,  C. Chu, J. Pu, S. Ren, Y. Wang, G. Zhong, T. K. Lu, C. Liu, and C. Zhong*, Diverse supramolecular nanofiber networks assembled by functional low-complexity domains. ACS Nano 2017, 11 (7), 6985–6995.

10.X. Y. Wang^#, Y. F. Li^#, C. Zhong*, Amyloid-directed assembly of nanostructures and functional devices for bionanoelectronics. Journal of Materials Chemistry B. 2015, 3, 4953-4958. (Invited review).

9.C. Zhong, T. K. Lu et al, Strong underwater adhesives made by self-assembling multi-protein nanofibres. Nature Nanotechnology 2014, 10, 858-866. (Highlighted in MIT News, the Scientist, Science Daily, Materials Today, MRS website and others)   

8.C. Zhong, Y. X. Deng, A. Kapetanovic, M. Rolandi, A polysaccharide bioprotonic field-effect transistor. Nature Communications 2011, 2, 476. (Highlighted by MIT Tech Review, IEEE Spectrum, MRS website and others).

7.C. Zhong, A.Kapetanovic, Y. X. Deng, M. Rolandi, A chitin nanofiber ink for airbrushing, replica molding and microcontact printing of self-assembled marco-, micro- and nanostructures. Advanced Materials 2011, 23, 4776-4781. (Inside cover feature).

6.C. Zhong, C.C.Chu, Biomimetic mineralization of acid polysaccharide-based hydrogels: Towards porous 3-dimensional bone-like biocomposites. Journal of Materials Chemistry 2012, 22, 6080-6087. (Highlighted in Biomimetic Materials Collection on RSC Biomaterials Science Blog).

5.C. Zhong, C. C. Chu et al., Synthesis, characterization and cytotoxicity of photo-crosslinked maleic chitosan-PEGDA hybrid hydrogels. Acta Biomaterialia, 2010, 6, 3908-3918.

3.C. Zhong, C. C. Chu, On the origin of amorphous cores in biomimetic calcium carbonate spherulites: new insights into spherulitic crystallization. Crystal Growth and Design 2010, 10, 5043-5049.

 Marco Rolandi*; Chao Zhong; Methods for the production of chitin nanofibers and uses thereof, 2014-12-16, US 14/123,726.

Chih-Chang Chu; Chao Zhong; Organo-soluble chitosan precursors and chitosan-derived biomaterials prepared thereof, 2011-6-23, PCT/US2009 /002017.

Lu, Timothy Kuan-Ta*; Zhong, Chao; Self-assembling underwater adhesives, 2019-10-22, US, 14540872.

钟超; 黄娇芳; 张琛; 刘苏莹; 枯草芽孢杆菌、生物膜及其构建和应用, 2016-12 -14, 中国, CN201611156490.X.